samboy, have a read of what everyone is saying first.
there are 2 things that you generally must have played with without changing internals of your carby which i assume you havent because you are asking if you have to turn the fuel of to take out the carby.
what sean01 is saying is what you should be doing, first check your valve clearances and then you have 2 screws on your carby which is what you have played with im guessing.
one of the screws adjusts what your bike will idle at - so obviously this could be too far out so when you turn the choke off the idle revs are too low to keep it going and bam the bike dies
the other screw is your fuel mixture screw and you could have played with this one, messing up the tune of the bike
i suggest adjusting the idle screw so that it revs even higher when the choke is on and then turn the choke off and see where it idles at. Once idling and warmed up, adjust the idle screw again to get the bike idling at the correct amount of revs that you want.